Name of the Procedure:

Infectious agent detection by nucleic acid (DNA or RNA); Trichomonas vaginalis, amplified probe technique

Summary

This procedure uses advanced molecular technology to detect the presence of Trichomonas vaginalis, a parasite causing trichomoniasis, by amplifying and analyzing its DNA or RNA. The technique is highly accurate, allowing for early and reliable diagnosis.

Purpose

The procedure is designed to identify Trichomonas vaginalis infections, which can cause uncomfortable symptoms and lead to complications if left untreated. Early detection leads to prompt and effective treatment, reducing the risk of transmission and complications.

Indications

• Symptoms of trichomoniasis such as unusual discharge, itching, and irritation.
• Patients with a history of sexually transmitted infections (STIs).
• Routine screening in sexually active individuals, especially women.

Preparation

• No specific preparation such as fasting is required.
• Patients should refrain from using vaginal douches or other intravaginal treatments 24 hours before the test.
• It's recommended to avoid sexual intercourse for 24 hours prior to the test.

Procedure Description

1. A sample is collected from the patient's genital area using a swab or urine sample.
2. The collected sample is then processed in a laboratory.
3. Using an amplified probe technique, the DNA or RNA of Trichomonas vaginalis is extracted and amplified.
4. Detection of the amplified genetic material confirms the presence of the infection.

No anesthesia or sedation is required for the sample collection.

Duration

The sample collection takes a few minutes. The analysis in the lab typically takes a few hours to a few days.

Setting

The sample collection is performed in a clinic, doctor's office, or healthcare facility. The analysis is conducted in a specialized laboratory.

Personnel

• The sample is usually collected by a nurse or a medical technician.
• Lab technicians or molecular biologists perform the amplification and analysis.

Risks and Complications

• Minor discomfort during sample collection.
• Very rare risk of an inaccurate result due to improper sample handling.

Benefits

• Accurate and early detection of Trichomonas vaginalis infection.
• Prompt treatment following a positive diagnosis.
• Reduction in the risk of complications and transmission to others.

Recovery

• No recovery needed as the procedure is non-invasive.
• Patients can resume normal activities immediately after the sample collection.
• Follow-up may involve discussing the results with a healthcare provider and starting appropriate treatment if the infection is detected.

Alternatives

• Wet mount microscopy: Less sensitive and requires immediate examination of a sample.
• Culture: More time-consuming but can also confirm the presence of the infection.

Patient Experience

Patients may experience minor discomfort during sample collection but no significant pain. Proper communication and gentle techniques used by healthcare providers help ensure comfort. Results are typically communicated within a few days along with next steps for treatment if needed.