Name of the Procedure:

Infectious Agent Detection by Nucleic Acid (DNA or RNA); Neisseria gonorrhoeae, Amplified Probe Technique

Summary

This test uses DNA or RNA technology to detect the presence of Neisseria gonorrhoeae, the bacteria responsible for gonorrhea, using an amplified probe technique. This method is extremely accurate and can detect even small amounts of bacterial genetic material.

Purpose

This procedure is used to diagnose infections caused by Neisseria gonorrhoeae. Early and accurate detection allows for appropriate treatment, preventing complications and reducing the risk of transmission.

Indications

• Symptoms of gonorrhea, such as unusual discharge, burning during urination, or pelvic pain.
• Routine screening for sexually transmitted infections (STIs) in sexually active individuals.
• Confirmation of suspected STI in patients with a sexual partner diagnosed with gonorrhea.

Preparation

• Avoid urination for at least one hour before the test to ensure an adequate sample.
• Inform your healthcare provider of any medications or supplements you are taking.
• No fasting or special diet is required.

Procedure Description

1. Sample Collection: A swab is taken from the affected area such as the throat, rectum, or genital area. Alternatively, a urine sample may be used.
2. Laboratory Analysis: The sample is sent to a lab where DNA or RNA amplification and probe technology are used to detect the presence of Neisseria gonorrhoeae.
3. Amplification: The genetic material of the bacteria, if present, is amplified to detectable levels.
4. Detection: Specialized probes then identify the amplified genetic material, confirming the presence of the bacteria.

Duration

The sample collection process takes only a few minutes. Laboratory analysis usually takes 1-3 days.

Setting

The sample can be collected in various settings including hospitals, outpatient clinics, and specialized STI clinics.

Personnel

• Primary care physician or nurse for sample collection.
• Laboratory technicians and microbiologists for sample analysis.

Risks and Complications

• Mild discomfort or irritation during sample collection.
• Rarely, slight bleeding after swab collection.
• No significant risks or complications are associated with the lab analysis itself.

Benefits

• Accurate and early detection of Neisseria gonorrhoeae.
• Timely treatment to prevent complications such as pelvic inflammatory disease or infertility.
• Reduced risk of spreading the infection to others.

Recovery

• The patient can resume normal activities immediately after sample collection.
• Follow any specific post-procedure instructions provided by your healthcare provider.

Alternatives

• Non-amplified probe techniques, which may be less sensitive.
• Culture tests, which can take longer to yield results but are useful in certain cases.
• Empirical treatment based on symptoms, though less precise without confirmation.

Patient Experience

• Mild discomfort during the swab collection, which is brief.
• Minimal to no discomfort with urine sample collection.
• Anxiety while waiting for results, which can be managed through counseling and information provided by healthcare professionals.
• Pain management is typically unnecessary as discomfort is minimal and short-lived.