Infectious agent detection by nucleic acid (DNA or RNA); Neisseria gonorrhoeae, direct probe technique
CPT4 code
Name of the Procedure:
Infectious Agent Detection by Nucleic Acid (DNA or RNA); Neisseria gonorrhoeae, Direct Probe Technique
Summary
This procedure involves detecting the genetic material (DNA or RNA) of Neisseria gonorrhoeae, the bacterium that causes gonorrhea, using a specialized probe technique. This method allows for the direct identification of the bacteria from a patient's sample.
Purpose
Medical Condition: Gonorrhea, a sexually transmitted infection Goals: The goal is to accurately and quickly identify the presence of Neisseria gonorrhoeae in order to facilitate prompt treatment and prevent complications or further transmission.
Indications
- Symptoms such as unusual discharge, pain during urination, or genital sores
- High-risk sexual activity
- Follow-up testing post-treatment
- Screening in pregnant women to prevent neonatal infection
Preparation
- No fasting required.
- Patients may be advised to avoid urinating one hour before the sample is collected.
- Inform the healthcare provider of any current medications or treatments.
Procedure Description
- Sample Collection: A healthcare provider collects a sample from the affected area (e.g., urine, swab from the genital area, throat, or rectum).
- Laboratory Processing:
- The sample is processed in a laboratory where the nucleic acids (DNA or RNA) are extracted.
- A probe containing a sequence of DNA or RNA complementary to the bacterial genetic material is added to the sample.
- If Neisseria gonorrhoeae DNA or RNA is present, it will bind to the probe, producing a detectable signal.
Tools/Equipment: Swabs, collection containers, nucleic acid extraction kits, probe detection systems Anesthesia/Sedation: Not required
Duration
The sample collection takes a few minutes. Laboratory analysis may take a few hours to a couple of days, depending on the facility.
Setting
- Outpatient clinic
- Laboratory
Personnel
- Nurse or technician for sample collection
- Laboratory technologist for sample analysis
- Healthcare provider for diagnosis and treatment discussion
Risks and Complications
- Minimal risk associated with sample collection
- Rare complications might include minor discomfort or irritation at the collection site
Benefits
- Rapid and accurate detection of Neisseria gonorrhoeae
- Enables prompt and appropriate treatment
- Reduces the risk of complications and transmission to others
Recovery
- No recovery time needed post-sample collection
- Follow healthcare provider’s recommendations for treatment if the test is positive
Alternatives
- Culture tests: Slower results, involves growing the bacteria in a lab.
- Rapid antigen tests: Generally less accurate but provide quicker results.
- Serology tests: Not typically used for active infection detection.
Patient Experience
- Mild discomfort during sample collection
- Minimal to no pain
- Anxiety relief with a prompt and accurate diagnosis
- If positive, peace of mind with effective treatment options and follow-up care