Name of the Procedure:

Oncology (melanoma), gene expression profiling by RTqPCR, PRAME and LINC00518, superficial collection using adhesive patch(es)

Summary

This procedure involves using a special sticky patch to collect skin cells from the surface of a suspected melanoma lesion. The collected cells are then tested using a technique called RTqPCR to measure the expression of two genes, PRAME and LINC00518, which can help in diagnosing melanoma.

Purpose

This procedure helps in the early detection and diagnosis of melanoma, a type of skin cancer. By measuring the levels of specific gene expressions, doctors can better understand the nature of a skin lesion and decide on appropriate treatment.

Indications

• Suspicious skin lesions that might be melanoma
• Previous inconclusive skin biopsies
• Patients with a family history of melanoma or other skin cancers

Preparation

• No special preparation is needed.
• Patients should ensure the area of skin to be tested is clean and free of any creams, lotions, or makeup.

Procedure Description

1. A healthcare professional will clean the area of the skin where the suspicious lesion is located.
2. An adhesive patch is placed over the lesion and pressed gently to ensure proper adhesion.
3. The patch is then removed, collecting skin cells from the surface of the lesion.
4. The collected cells are sent to a lab where RTqPCR is performed to measure the levels of PRAME and LINC00518 gene expression.
5. Results are analyzed and discussed with the patient to determine the next steps.

No anesthesia or sedation is needed, as the procedure is non-invasive and painless.

Duration

The collection process typically takes about 10-15 minutes. Lab analysis can take a few days.

Setting

The procedure is performed in an outpatient clinic or a dermatologist's office.

Personnel

• Dermatologist or trained healthcare professional for the patch application
• Laboratory technicians for gene expression analysis

Risks and Complications

• Minimal risk, mainly skin irritation from the adhesive patch
• Rarely, inaccurate results due to insufficient cell collection, requiring a repeat procedure

Benefits

• Non-invasive and painless
• Provides crucial information for the early detection of melanoma
• Quick procedure with no recovery time

Recovery

• No specific recovery is needed
• Patients can resume normal activities immediately
• Follow-up appointment for discussing lab results

Alternatives

• Traditional skin biopsies, which are more invasive
• Dermatoscopic examination, which may not provide as much detailed molecular information

Pros of this procedure:

• Non-invasive, quick, and painless

Cons of alternatives:

• Biopsies can be painful and require healing time
• Dermatoscopic examination may not be as conclusive

Patient Experience

During the procedure, the patient will feel the adhesive patch being applied and removed from their skin, which is generally comfortable. After the procedure, normal activities can be resumed immediately. Any minor skin irritation from the patch will usually resolve quickly.