Infectious agent antigen detection by immunoassay technique, (eg, enzyme immunoassay [EIA], enzyme-linked immunosorbent assay [ELISA], immunochemiluminometric assay [IMCA]) qualitative or semiquantitative, multiple-step method; Chlamydia trachomatis
CPT4 code
Name of the Procedure:
Infectious Agent Antigen Detection by Immunoassay Technique
Common names: EIA, ELISA, IMCA
Technical term: Enzyme Immunoassay, Enzyme-Linked Immunosorbent Assay, Immunochemiluminometric Assay - Chlamydia trachomatis
Summary
This procedure uses immunoassay techniques, such as EIA, ELISA, or IMCA, to qualitatively or semiquantitatively detect antigens of Chlamydia trachomatis, a common sexually transmitted infection. The test involves multiple steps to identify the presence of the Chlamydia bacteria in a sample.
Purpose
This test identifies the presence of Chlamydia trachomatis antigens to diagnose Chlamydia infections. Early detection can lead to effective treatment, reducing the risk of complications such as pelvic inflammatory disease (PID) and infertility.
Indications
- Symptoms such as unusual genital discharge, burning during urination, or pain during intercourse.
- Routine screening for sexually active individuals, especially those with multiple partners.
- Pregnant women to prevent newborn infections.
- Follow-up testing after treatment to ensure the infection has cleared.
Preparation
- No fasting or special diet required.
- Refrain from urinating for at least one hour before the test if a urine sample is used.
- Women may need to avoid douching or using vaginal creams for 24 hours prior.
- Inform your healthcare provider of any medications or supplements you're taking.
Procedure Description
- Sample Collection: A sample is collected via a swab from the cervix (for women), urethra (for men), or urine.
- Sample Processing: The sample is processed in the lab using EIA, ELISA, or IMCA techniques.
- Detection: The immunoassay technique involves binding the antigens to antibodies on a plate, adding enzymes or other markers to identify the antigens, and detecting these markers through various methods like color change (EIA/ELISA) or light emission (IMCA).
Tools/Equipment: Swabs, collection containers, lab plates, assay kits.
Anesthesia/Sedation: None required.
Duration
The actual sample collection takes a few minutes. Laboratory processing and results can take several hours to a few days.
Setting
The sample collection can be performed in a doctor's office, clinic, or hospital. Laboratory analysis occurs in a specialized lab.
Personnel
- Sample collected by a nurse or doctor.
- Laboratory processing by medical laboratory technologists or technicians.
Risks and Complications
- Mild discomfort or irritation at the sample collection site.
- Very rare risk of incorrect results (false positives/negatives).
Benefits
- Quick and accurate detection of Chlamydia trachomatis.
- Early diagnosis leads to prompt treatment, reducing the risk of serious complications.
Recovery
- No significant recovery time needed.
- Normal activities can be resumed immediately after sample collection.
- Follow healthcare provider’s instructions regarding treatment if the test is positive.
Alternatives
- Nucleic acid amplification tests (NAATs), which are highly sensitive for detecting Chlamydia.
- Culture tests, which can take longer for results but are highly specific.
Pros and Cons of Alternatives:
- NAATs: More sensitive but typically more expensive.
- Culture: Highly accurate but slower and more labor-intensive.
Patient Experience
- During the sample collection, you may experience mild discomfort or pressure.
- There may be light spotting or cramping for women after a cervical swab.
- Post-procedure, there is generally no pain, and normal activities can be continued immediately.
- Any positive results will be discussed with you for further treatment options.