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Thawing of cryopreserved; sperm/semen, each aliquot

CPT4 code

Name of the Procedure:

Thawing of Cryopreserved Sperm/Semen, each aliquot


Thawing of cryopreserved sperm/semen is the process of warming frozen sperm samples to restore their viability for use in fertility treatments, such as intrauterine insemination (IUI) or in vitro fertilization (IVF).


The procedure addresses infertility issues by providing viable sperm for assisted reproductive techniques. The goal is to allow conception by using previously collected and frozen sperm, either from a partner or a donor.


  • Male infertility issues (e.g., azoospermia)
  • Prior to cancer treatment that may affect fertility
  • Use of donor sperm
  • Synchronization with the female partner's ovulation for assisted reproduction


  • No specific pre-procedure instructions for the patient.
  • A consent form may be needed.
  • Coordination with the timing of the female partner’s ovulation or treatment cycle.

Procedure Description

  1. The frozen sperm sample is retrieved from cryostorage.
  2. The sample is placed in a controlled environment to ensure gradual thawing.
  3. It is then submerged in a warm water bath for a specified period.
  4. Post-thaw, the sample is assessed for viability, motility, and concentration.
  5. The sperm is prepared for use in fertility treatments according to the specific needs of the patient.


  • Cryostorage tank
  • Thawing bath
  • Microscopy equipment for assessment

Anesthesia: Not applicable.


Typically takes around 30-60 minutes.


Performed in a fertility clinic or a specialized laboratory.


  • Laboratory technician
  • Reproductive endocrinologist (may be involved in subsequent fertility treatment)

Risks and Complications

  • Reduced sperm viability or motility post-thaw. Management: Additional thawed aliquots or alternative fertility treatments.


  • Provides the opportunity for conception using stored sperm.
  • Facilitates family planning and fertility preservation.


  • No recovery time needed.
  • Follow any specific instructions provided for subsequent fertility treatment.


  • Fresh sperm collection. Pros: Potentially higher motility and viability. Cons: May not be feasible for all patients (e.g., post-chemotherapy, absence of sperm).

Patient Experience

The patient will not feel or experience anything directly during the thawing procedure itself. Pain and discomfort associated with subsequent fertility treatments should be discussed with the healthcare provider.

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