Name of the Procedure:

Amines, vaginal fluid, qualitative
Also known as the "amine test" or "whiff test."

Summary

The amine test is a simple, quick diagnostic procedure to detect the presence of certain bacteria in vaginal fluid. A small sample of vaginal fluid is mixed with a chemical solution, and a distinct fishy odor indicates a positive result for bacterial vaginosis.

Purpose

This procedure helps diagnose bacterial vaginosis, a common infection caused by an imbalance of bacteria in the vagina. Proper diagnosis can help target and treat the infection effectively.

Indications

• Symptoms such as unusual vaginal discharge, itching, or odor.
• Suspected bacterial vaginosis based on a physical examination.
• Pregnant women experiencing these symptoms or at higher risk of infection.

Preparation

• No special preparation is usually required.
• Patients may be advised to avoid vaginal douching or intercourse 24 hours before the test.
• Inform the healthcare provider of any medications being taken.

Procedure Description

1. The healthcare provider collects a small sample of vaginal fluid using a sterile swab.
2. The sample is placed on a slide or in a small container.
3. A few drops of a 10% potassium hydroxide (KOH) solution are added to the sample.
4. The provider assesses for the release of a fishy odor, indicating a positive result.

Duration

The procedure typically takes about 5-10 minutes.

Setting

Performed in a doctor's office, outpatient clinic, or gynecological exam room.

Personnel

• Healthcare provider (e.g., gynecologist, nurse practitioner, or physician assistant).
• Medical assistant may assist with sample collection.

Risks and Complications

• Minimal risk involved.
• Rare cases of mild discomfort during sample collection.
• No major complications associated with the test.

Benefits

• Quick and accurate diagnosis of bacterial vaginosis.
• Immediate results to guide appropriate treatment.
• Helps prevent complications related to untreated bacterial vaginosis.

Recovery

• No recovery period needed.
• Patients can resume normal activities immediately after the test.
• Follow the healthcare provider’s recommendations if treatment is needed.

Alternatives

• Microscopic examination of vaginal fluid (wet mount).
• DNA testing or cultures for bacterial identification.
• Pros: The KOH whiff test is quick and cost-effective.
• Cons: Other tests may provide more detailed information about the specific bacteria present.

Patient Experience

• Mild discomfort may be experienced during the collection of the vaginal fluid sample.
• The test itself is painless and quick.
• The provider may discuss the results and next steps immediately following the test.