Morphometric analysis, in situ hybridization (quantitative or semi-quantitative), manual, per specimen; each multiplex probe stain procedure
CPT4 code
Name of the Procedure:
Morphometric analysis, in situ hybridization (quantitative or semi-quantitative), manual, per specimen; each multiplex probe stain procedure.
Summary
This procedure involves analyzing and measuring specific DNA or RNA sequences in tissue samples using detailed staining techniques. It helps identify the presence and quantity of genetic material inside cells, aiding in various diagnostic processes.
Purpose
The procedure is used to detect and measure specific genetic sequences within tissue samples. It is commonly employed in the diagnosis and study of genetic disorders, cancer, and other diseases at the molecular level. Goals include providing precise genetic information that can guide treatment plans and improve diagnostic accuracy.
Indications
- Suspected genetic disorders
- Cancer diagnosis and staging
- Monitoring disease progression or response to treatment
- Research purposes in genetics and molecular biology
Preparation
- Follow any fasting or medication instructions provided by your healthcare team.
- You may need blood tests or imaging studies prior to the procedure.
- Inform your doctor about any allergies or existing medical conditions.
Procedure Description
- A tissue sample is collected, typically through a biopsy.
- The sample is prepared and placed on a slide.
- Specific probes that bind to target DNA or RNA sequences are applied to the tissue.
- These probes are labeled with fluorescent or chromogenic markers.
- The sample is examined under a microscope to identify and quantify the genetic material.
- Analysis is done manually, meaning a specialist interprets the results by direct observation.
Tools and equipment:
- Microscopes
- Fluorescent or chromogenic probes
- Tissue sample slides
Anesthesia: Typically, no anesthesia or sedation is needed for the analysis itself; however, local anesthesia may be used during the biopsy for sample collection.
Duration
The entire procedure, including preparation and analysis, can take several hours to a day, depending on the complexity and number of specimens.
Setting
The procedure is usually performed in a laboratory setting within a hospital, research facility, or specialized clinic.
Personnel
- Pathologist or molecular biologist
- Lab technicians
- Histotechnologists
Risks and Complications
- Minimal risks associated with the biopsy procedure for sample collection (e.g., infection, bleeding)
- Interpretation errors, though rare, can occur if the sample is not properly prepared or analyzed.
Benefits
- Provides detailed genetic information that can guide treatment and improve outcomes.
- Assists in accurate diagnosis and monitoring of diseases.
- Helps identify specific genetic changes that may be targets for therapy.
Recovery
- No special recovery needed from the analysis itself.
- Follow any post-biopsy care instructions provided (e.g., wound care, managing discomfort).
Alternatives
- PCR (Polymerase Chain Reaction) techniques
- Next-generation sequencing
- Immunohistochemistry Each alternative has its pros and cons in terms of specificity, sensitivity, cost, and time required.
Patient Experience
- The analysis itself is non-invasive beyond the initial tissue sampling.
- Patients may experience minor discomfort from the biopsy.
- Pain management, if necessary, is typically straightforward with local anesthesia and over-the-counter pain relievers.
Proper communication with healthcare providers will ensure optimal care and accurate results.