87336 Infectious agent antigen detection by immunoassay technique, (eg, enzyme immunoassay [EIA], enzyme-linked immunosorbent assay [ELISA], immunochemiluminometric assay [IMCA]) qualitative or semiquantitative, multiple-step method; Entamoeba histolytica dispa

Name of the Procedure:

Infectious Agent Antigen Detection by Immunoassay Technique (e.g., Enzyme Immunoassay [EIA], Enzyme-Linked Immunosorbent Assay [ELISA], Immunochemiluminometric Assay [IMCA]) for Entamoeba histolytica

Summary

This test detects the presence of specific antigens from the parasitic organism Entamoeba histolytica in a patient's stool sample. Entamoeba histolytica is known to cause amebiasis, which can lead to gastrointestinal symptoms. The test can be qualitative (indicating whether the antigen is present or not) or semiquantitative (providing an estimate of the amount of antigen present).

Purpose

This procedure aims to diagnose infections caused by Entamoeba histolytica. Accurate detection helps guide appropriate treatment to alleviate symptoms and prevent complications associated with the infection.

Indications

• Persistent diarrhea or dysentery
• Abdominal pain and cramping
• Bloody stools
• History of travel to areas where Entamoeba histolytica is prevalent
• Patients with compromised immune systems experiencing gastrointestinal symptoms

Preparation

• No special preparation is typically required.
• Patients may need to provide multiple stool samples over a few days.
• Patients should inform their healthcare provider about any medications they are taking, as certain drugs may affect the test results.

Procedure Description

1. A stool sample is collected from the patient using a clean container provided by the healthcare provider.
2. The sample is sent to the laboratory, where it undergoes multiple steps involving reagents and incubation periods.
3. The sample is exposed to antibodies that specifically bind to Entamoeba histolytica antigens.
4. If the antigens are present, a detectable signal is produced, measured, and analyzed using various assays (e.g., EIA, ELISA, IMCA).
5. Results are interpreted as either positive (antigen present) or negative (antigen not present), with semiquantitative methods providing an estimate of antigen levels.

Duration

The laboratory processing typically takes a few hours, but results may be available within 1-2 days, depending on the lab's workload and protocols.

Setting

The test is performed in a specialized laboratory setting. Sample collection can be done in a clinic, hospital, or at the patient’s home with proper instructions.

Personnel

• Laboratory technicians or medical technologists perform the test.
• Physicians interpret the results and discuss them with the patient.

Risks and Complications

• There are no significant risks associated with the stool sample collection.
• In rare cases, the sample may be contaminated, leading to inaccurate results, necessitating a repeat test.

Benefits

• Accurate diagnosis of Entamoeba histolytica infection
• Prompt and appropriate treatment can be initiated
• Prevents complications such as severe dehydration, intestinal damage, and abscess formation

Recovery

• No recovery is required since the procedure is non-invasive.
• Patients can resume normal activities immediately after providing the stool sample.

Alternatives

• Microscopic examination of stool for cysts and trophozoites, though less sensitive and specific.
• Polymerase Chain Reaction (PCR) test for Entamoeba histolytica DNA, more sensitive but less commonly available.

Patient Experience

• The patient will need to collect a stool sample, which might be uncomfortable but is not painful.
• There is minimal physical discomfort involved in the sample collection.
• Any questions or concerns about the procedure can be addressed by the healthcare provider to ensure patient comfort and understanding.