IGK@ (Immunoglobulin kappa light chain locus) (eg, leukemia and lymphoma, B-cell), gene rearrangement analysis, evaluation to detect abnormal clonal population(s)
CPT4 code
Name of the Procedure:
IGK@ (Immunoglobulin kappa light chain locus) Gene Rearrangement Analysis, Evaluation to Detect Abnormal Clonal Population(s)
Summary
This genetic test looks for specific changes in the DNA of B-cells, which are a type of white blood cell. These changes can indicate the presence of certain blood cancers such as leukemia or lymphoma. By identifying abnormal cell populations, doctors can diagnose and plan treatment for these diseases.
Purpose
The procedure is primarily used to diagnose blood cancers such as leukemia and lymphoma. It helps to identify abnormal clonal populations of B-cells that are indicative of these conditions. The goal is to detect these changes early so that appropriate treatment can be started as soon as possible.
Indications
- Symptoms such as unexplained bruising, frequent infections, or feeling very tired.
- Abnormal blood test results suggesting a possible blood cancer.
- Family history of leukemia or lymphoma.
- Monitoring response to treatment in patients already diagnosed with B-cell malignancies.
Preparation
- No special preparation is typically needed.
- Your doctor may order other blood tests or imaging studies beforehand.
- Inform your healthcare provider of any medications you are taking.
Procedure Description
- Sample Collection: A blood sample is typically collected from a vein in your arm. In some cases, a bone marrow sample might be needed.
- Laboratory Analysis: The sample is sent to a laboratory where DNA is extracted and examined for specific gene rearrangements in the immunoglobulin kappa light chain locus.
- Evaluation: The lab uses specialized techniques such as polymerase chain reaction (PCR) to detect the presence of clonal populations.
Duration
The sample collection takes a few minutes. Laboratory analysis can take several days to a few weeks.
Setting
The blood or bone marrow sample collection is usually done in a hospital or outpatient clinic. Laboratory analysis is conducted at a specialized diagnostic lab.
Personnel
- Phlebotomist or nurse for sample collection.
- Laboratory technologists for analysis.
- Pathologist or hematologist to interpret the results.
Risks and Complications
- Minor bleeding or bruising at the sample collection site.
- Infection at the puncture site (rare).
- Inaccurate results if the sample is not handled or processed correctly (mitigated by following strict laboratory protocols).
Benefits
- Early detection and diagnosis of blood cancers like leukemia and lymphoma.
- Helps in planning the most effective treatment strategy.
- All results are generally specific, allowing for accurate diagnosis.
Recovery
- Minimal recovery time is needed after sample collection.
- Normal activities can usually be resumed immediately.
- Follow-up appointments may be scheduled to discuss the results and next steps.
Alternatives
- Other genetic testing methods.
- Imaging studies such as PET or CT scans.
- Bone marrow biopsy.
- Each alternative has its pros and cons in terms of accuracy, invasiveness, and cost.
Patient Experience
- You may feel a brief pinch when the blood sample is taken.
- Slight soreness or bruising is possible at the puncture site.
- Generally, there is minimal discomfort, and any pain or bruising should resolve quickly.